Immunological biomarkers of the vitreous responsible for proliferative alteration in the different forms of retinal detachment.

BACKGROUND: The purpose of the study was to explore the immunological components that are responsible for the proliferative alterations in the different forms of retinal detachment (RD). METHODS: Vitreous fluids were collected during 23G pars plana vitrectomy from 54 eyes of 54 patients with different RD types, such as rhegmatogenous RD (RRD) without proliferative vitreoretinopathy (PVR) (n = 30), PVR (n = 16) and proliferative diabetic retinopathy (PDR) with tractional RD (n = 8). Vitreous fluids were obtained from 19 eyes with epiretinal membrane (ERM), which were used as control samples. A multiplex chemiluminescent immunoassay was performed to evaluate the concentrations of 48 cytokines, chemokines and growth factors. RESULTS: The expression levels of eotaxin, IFN-gamma, IL-6, IL-8, IL-16, MCP-1, MIF and MIP-1 beta were significantly higher in all RD groups than in the ERM group. The levels of CTACK, IP-10, SCGF-beta, and SDF-1 alpha were significantly higher in patients with diabetic tractional RD and PVR than in other patients. The upregulation of VEGF and IL-18 was detected in PDR. CONCLUSIONS: Our results indicate that complex and significant immunological mechanisms are associated with the pathogenesis of different forms of RD: selected cytokines, chemokines and growth factors are upregulated in the vitreous of eyes with RD. The detected proteins are present in different concentrations both in RRD and PVR. In the presence of PVR and PDR, the majority of cytokines are upregulated; thus, they may serve as biomarkers to estimate the progression or severity level of proliferation and later to develop personalized therapeutic strategies to slow down or prevent pathological changes.

Position of macula lutea and presence of proliferative vitreoretinopathy affect vitreous cytokine expression in rhegmatogenous retinal detachment.

Our purpose was to evaluate the concentrations of vitreous cytokines in patients with rhegmatogenous retinal detachment (RRD). We hypothesized that patients with macula on RRD have lower levels of cytokines compared to patients with macula off RRD and proliferative vitreoretinopathy (PVR). Vitreous fluids were collected during 23G pars plana vitrectomy from 58 eyes of 58 patients. Indication for vitrectomy included macula off and macula on RRD, PVR, and idiopathic epiretinal membrane (ERM). A multiplex chemiluminescent immunoassay was performed to measure the concentrations of 48 cytokines, chemokines, and growth factors. Levels of HGF, IL-6, IL-8, IL-16, IFN-gamma, MCP-1, and MIF were significantly higher in all groups of retinal detachment compared to ERM. Levels of CTACK, eotaxin, G-CSF, IP-10, MIG, SCF, SCGF-beta, SDF-1alpha were significantly higher in PVR compared to macula on RRD and ERM. Levels of IL-1ra, IL-5, IL-9, M-CSF, MIP-1alpha, and TRIAL were significantly higher in PVR compared to macula on RRD. Our results indicate that the position of macula lutea and the presence of PVR significantly influence vitreous cytokine expression. The detected proteins may serve as biomarkers to estimate the possibility of PVR formation and may help to invent personalized therapeutic strategies to slow down or prevent PVR.

[Metastatic endophthalmitis following lung transplantation]. / Metasztatikus endophthalmitis tüdotranszplantáción atesett betegekben.

Intraocular septic--bacterial, viral or fungal--metastasis originating from an infectious focus is very rare but serious complication. The authors performed one enucleation and one evisceration on two patients because of endophthalmitis secondary to pulmonary infection following lung transplantation. The pathogens were Pseudomonas aeruginosa and Mycobacterium xenopi respectively. With this case report the authors aim to draw attention to a rare, but serious ophthalmic complication of organ transplantation: endophthalmitis; its early care, to help prevent the need for enucleation.

[Role of the vitreous body in vitreoretinal diseases]. / Az üvegtest szerepe a vitreoretinalis betegségekben.

Recent research during the last decades revealed evidence for the pathogenic role of the vitreous in many diseases of the retina. After a survey of the surgical anatomy and biochemistry of the vitreous, the author summarises present knowledge of various vitreoretinal disorders. Vitreoretinal disorders are discussed as follows: congenital anomalies, pathologic vitreoretinal adhesions, vitreous opacities, inflammatory diseases, vitreous hemorrhages and cellular infiltration of the vitreous. Special emphasis is placed on vitroretinal disorders of general importance, such as diabetic retinopathy, endogenous (fungal) endophthalmitis, Whipple disease, Crohn disease, sarcoidosis, shaken-baby syndrome, Terson syndrome, metastatic tumours of the choroid, cellular infiltration of the vitreous due to leukaemia or non-Hodgkin lymphoma, and amyloidosis. During the last few years better understanding of vitreoretinal disorders and improved diagnostic and therapeutic methods opened a new era of vitreoretinal surgery.